Record ID No. |
6152 |
Author(s) |
Wei Y.*, Chen Z. P., Wu F. C., Li J. N., Guan Y. X. S., Li F. S., Zeng Q. R. and Hou H. , 2015 |
Affiliation |
*State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, P.R. China. |
Title |
Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb mining. |
Source. Vol.(no):Page |
Journal of Microbiology and Biotechnology. 25(8): 1202-1215. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Ecology |
Sub-subjects |
Biodiversity Heavy Metals |
Host |
Boehmeria nivea |
Organism |
Glomus spp., Acaulospora spp., Funneliformis spp., Claroideoglomus spp., Gigaspora spp., Paraglomus spp., Rhizophagus spp., Sclerocystis spp., Scutellospora spp.. |
Country |
China |
Abstracts |
Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is
available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this study was to investigate the
colonization and molecular diversity of AMF associated with the Sb accumulator ramie(Boehmeria nivea) growing in Sb-contaminated soils. Four Sb mine spoils and one adjacent reference area were selected from Xikuangshan in southern China. PCR-DGGE was used to analyze the AMF community composition in ramie roots. Morphological identification was
also used to analyze the species in the rhizosphere soil of ramie. Results obtained showed that mycorrhizal symbiosis was established successfully even in the most heavily polluted sites. From the unpolluted site Ref to the highest polluted site T4, the spore numbers and AMF diversity increased at first and then decreased. Colonization increased consistently with the
increasing Sb concentrations in the soil. A total of 14 species were identified by morphological analysis. From the total number of species, 4 (29%) belonged to Glomus, 2 (14%) belonged to Acaulospora, 2 (14%) belonged to Funneliformis, 1 (7%) belonged to Claroideoglomus, 1 (7%)
belonged to Gigaspora, 1 (7%) belonged to Paraglomus, 1 (7%) belonging to Rhizophagus, 1 (7%) belonging to Sclervocystis, and 1 (7%) belonged to Scutellospora. Some AMF sequences were
present even in the most polluted site. Morphological identification and phylogenetic analysis both revealed that most species were affiliated with Glomus, suggesting that Glomus was the
dominant genus in this AMF community. This study demonstrated that ramie associated with AMF may have great potential for remediation of Sb-contaminated soils. |