Record ID No. |
620 |
Author(s) |
Andrea Caroline Ruthes, Elaine R. Carbonero, Marina Machado Córdova, Cristiane Hatsuko Baggio, Adair Roberto Soares Santos, Guilherme Lanzi Sassaki, Thales Ricardo Cipriani, Philip Albert James Gorin, Marcello Iacomini , 2013 |
Affiliation |
Departamento de BioquÃmica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba, PR, Brazil, email:(Marcello Iacomini) iacomini@ufpr.br |
Title |
Lactarius rufus (1→3),(1→6)-β-d-glucans: Structure, antinociceptive and anti-inflammatory effects |
Source. Vol.(no):Page |
Carbohydrate Polymers, 94(1): 129-136p. |
Categories |
Ectomycorrhiza |
Subjects |
Biochemistry |
Sub-subjects |
Carbohydrate metabolism |
Host |
Ectomycorrhiza |
Organism |
Lactarius rufus |
Country |
Brazil, S. America |
Abstracts |
Medicinal health benefits uses of edible as well as non-edible mushrooms have been long recognized. The pharmacological potential of mushrooms, especially antitumor, immunostimulatory and anti-inflammatory activities has been documented. Wild ectomycorrhizal mushroom, Lactarius rufus had the anti-inflammatory and antinociceptive potential of their polysaccharides evaluated using the formalin model. Two structurally different (1→3),(1→6)-linked β-d-glucans were isolated from fruiting bodies. Soluble (FSHW) β-d-glucan 1–30 mg kg−1 produced potent inhibition of inflammatory pain caused by formalin when compared with the insoluble one (IHW), suggesting that solubility and/or branching degree could alter the activity of β-glucans. Their structures were determined using mono- and bi-dimensional NMR spectroscopy, methylation analysis, and controlled Smith degradation. They were β-d-glucans, with a main chain of (1→3)-linked Glcp residues, substituted at O-6 by single-unit Glcp side chains (IHW), on average to every fourth residue of the backbone, or by mono- and few oligosaccharide side chains for soluble β-glucan. |