Record ID No. |
6208 |
Author(s) |
Andrango C.*, Cueva M., Viera W. and Duchicela J. , 2016 |
Affiliation |
*Department of Life Sciences and Agriculture, Universidad de las Fuerzas, Armadas – ESPE, P.O.BOX 1715231B, Av. General Rumiñahui s/n, Sangolquí, Ecuador. |
Title |
Evaluation of methods to estimate mycorrhizal inoculum potential in field soils. |
Source. Vol.(no):Page |
Science Magazine. 18(3): 329-352. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Methodology |
Host |
NA |
Organism |
NA |
Country |
Ecuador |
Abstracts |
Arbuscular Mycorrhizal Fungi constitute a group of microorganisms that form symbiotic relationships with plant roots in most ecosystems. Mycorrhizal potential or inoculum potential (IP) are terms used in many studies as an indicator of propagule density and mycorrhizal activity in the soil. It allows the quality and infectivity of soil inoculum to be evaluated and is used as a biological indicator. A number of methods have been used traditionally to assess the mycorrhizal potential to colonize a plant. These methods include: Infection Unit (IU), Mean Infection Percentage (MIP), Most Probable Number (MPN) and Mycorrhizal Soil Infectivity (MSI), and others. This study reviews the state of the art of these methodologies and discusses pros and cons, and how the methodologies can be implemented and applied most effectively. We evaluated three techniques (IU, MIP and MPN) varying the field soil types as inoculum, plant hosts and proportions of substrate to inoculum (w/w). The statistical analysis detected that the treatment with the factors combining: MIP x Corn x Proportion 4:1 of Clay soil to inoculum was the most significant (F=2.41, p=0.0915). Hence, the data presented in this paper should allow researchers to select an effective method for assessing AM inoculum potential. |