Record ID No. |
640 |
Author(s) |
Stewart, L.I., Jabaji-Hare, S., Driscoll, B.T* , 2006 |
Affiliation |
*McGill University, Microbiology Unit, Department of Natural Resources Science, 21111 Lakeshore Rd, Ste Anne De Bellevue, PQ H9X 3V9, Canada |
Title |
Effects of external phosphate concentration on glucose-6-phosphate dehydrogenase gene expression in the arbuscular mycorrhizal fungus Glomus intraradices |
Source. Vol.(no):Page |
Canadian journal of microbiology. 52(9):823-830p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Soil plant relations |
Sub-subjects |
Nutrition |
Host |
n.a. |
Organism |
Glomus intraradices |
Country |
Canada, N. America |
Abstracts |
Specific primers were developed to amplify a 227 bp segment of the arbuscular mycorrhizal fungus Glomus intraradices gene encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme involved in the pentose phosphate pathway. G6PDH gene expression was measured by real-time quantitative reverse transcriptase - polymerase chain reaction in response to phosphorus (P) concentrations in the growth medium of colonized transformed carrot roots. We investigated the effects of different P concentration treatments on carbon (C) metabolism within the intraradical mycelia of G. intraradices. The results showed a significant (P = 0.017) down-regulation of G6PDH expression in the intraradical mycelia of G. intraradices cultures grown in high P than low P conditions but no significant difference in regulation in excessive P concentrations when compared with the low P or high P concentrations. These results indicate that a reduction in the C flow from the host could be occurring as a result of elevated P and that a decrease in fungal G6PDH gene expression occurs, but not in the short term (less than 2 h). Reduced C flow from the host could lead to reduced fungal growth and root colonization, as was observed under high soil P conditions. |