Record ID No. |
65 |
Author(s) |
Krause K., Kothe E. , 2006 |
Affiliation |
University of Jena, Department of Microbiology, Neugasse 25, D-07743 Jena, Germany |
Title |
Use of RNA fingerprinting to identify fungal genes specifically expressed during ectomycorrhizal interaction |
Source. Vol.(no):Page |
Journal of basic microbiology.46(5):387-399p. |
Categories |
Ectomycorrhiza |
Subjects |
Genetics |
Host |
Picea abies |
Organism |
Tricholoma vaccinum |
Country |
Germany, Europe |
Abstracts |
The ecosystem soil is characterized by interactions
between microorganisms and plants including mycorrhiza -
mutualistic interactions between fungi and plant roots. Species
of the basidiomycete genus Tricholoma form ectomycorrhiza with
tree roots which is characterized by morphological and metabolic
changes of both partners, yet molecular mechanisms of the
interaction are poorly understood. We performed differential
display with arbitrarily primed RT-PCR using ectomycorrhiza
between the basidiomycete Tricholoma vaccinum and its compatible
host spruce (Picea abies) to isolate mycorrhiza-specific fungal
gene fragments. 76 differentially expressed PCR fragments were
verified and checked for plant or fungal origin and expression
pattern. Of 20 fungal fragments with mycorrhiza-specific
expression, sequence analyses were performed to identify
homologs with known function of the encoded protein. Among the
genes identified were orthologs to an aldehyde dehydrogenase, an
alcohol dehydrogenase and a protein of the MATE transporter
family, all with possible function in plant pathogen response. A
phospholipase B, a beta-glucosidase and a binding protein of
basic amino acids might play a role in nutrient exchange and
growth in planta. A protein similar to inactive E2 compounds of
ubiquitin-conjugating enzymes like CROC-1 and MMS2, a Ras
protein and an APS kinase were placed in signal transduction and
two retrotransposons of the Ty3-gypsy and the Ty1-copia family
are expressed most likely due to stress.
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