Record ID No. |
653 |
Author(s) |
Timonen, S., Drew, E., Smith, S.E , 2006 |
Affiliation |
University of Adelaide, School of Earth & Environmental Sciences, Soil & Land System, Waite Campus, Adelaide, SA 5005, Australia |
Title |
Effect of cytoskeletal inhibitors on mycorrhizal colonisation of tomato roots |
Source. Vol.(no):Page |
Symbiosis. 41(2):81-86p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
General |
Host |
Solanum esculentum |
Organism |
Glomus intraradices, G. mosseae |
Country |
Australia |
Abstracts |
The role of the plant cytoskeletal filaments in arbuscular mycorrhizal (AM) colonisation was evaluated by disrupting actin filaments and microtubules with Cytochalasin D or Oryzalin. Two genotypes of tomato (Solanum esculentum L.), wild-type 76R and a mutant with reduced AM colonisation (rmc), and three different AM fungi with different colonisation phenotypes in the mutant, were used in order to better understand the events controlling AM colonisation of roots. Disruption of plant microtubules of the wild-type tomatoes did not have a clear effect on AM colonisation or hyphal growth of any of the fungal strains used. However, disruption of plant actin filaments reduced colonisation, as well as formation of arbuscules, particularly with Glomus intraradices and G. mosseae. Application of plant cytoskeleton inhibitors to the rmc mutant tomato did not increase mycorrhizal colonisation in either case. These results imply that the plant cytoskeleton is involved in mycorrhiza development in plant root cells, but is not involved in the primary mechanisms that exclude the fungi from the roots of this tomato mutant or limit intracellular development. |