Record ID No. |
6555 |
Author(s) |
Declerck S.*, D'Or D., Bivort C. and de Souza F. A. , 2004 |
Affiliation |
*Université Catholique de Louvain, Mycothèque de l'Université catholique de Louvain (MUCL), Unité de microbiologie, 3 Place Croix du Sud, 1348 Louvain-La-Neuve, Belgium. |
Title |
Development of extraradical mycelium of Scutellospora reticulata under root-organ culture: spore production and function of auxiliary cells. |
Source. Vol.(no):Page |
Mycological Research. 108(1): 84-92. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Physiology |
Host |
NA |
Organism |
Scutellospora reticulata |
Country |
Belgium |
Abstracts |
The development of the extraradical mycelium and auxiliary cells and spore production of Scutellospora reticulata in association with Ri T-DNA transformed carrot roots was followed under root-organ culture conditions. Extraradical mycelium development followed classical lag-exponential-plateau phases, with an additional late decline phase in number of auxiliary cells. Spore production started in parallel with a critical extraradical mycelium biomass produced, continued long after root growth ceased and during the late decline in auxiliary cells number. Isolated auxiliary cells were shown to exhibit hyphal re-growth, but not root colonization, either in situ or in vitro. These results showed that root and extraradical mycelium development were intimately associated in a sequence where both grew together during active root growth, followed during root aging by a period in which only the fungus developed. Spore production appeared dependent on a critical extraradical mycelium biomass and on the re-allocation of resources from both the intraradical mycelium and the auxiliary cells via the hyphal network. |