Record ID No. |
6689 |
Author(s) |
Lin L. C.*, Chang T. P., Tsai C. H. and Huang H. N. , 2016 |
Affiliation |
*Department of Forestry and Natural Resources, National Chiayi University, Chiayi, 60004 Taiwan, Republic of China. |
Title |
Characterization of Pisolithus orientalis from Taiwan and its Compatibility with Cyclobalanopsis glauca. |
Source. Vol.(no):Page |
Research Journal of Microbiology. 11(6): 194-201. |
Categories |
Ectomycorrhiza |
Subjects |
Biochemistry Morphology Mycorrhizal Synthesis Physiology |
Host |
Cyclobalanopsis glauca |
Organism |
Pisolithus orientalis |
Country |
China |
Abstracts |
The mycorrhizae of most Fagaceae species is known to be ectomycorrhizae. The objective of this study was to investigate the colony morphology, growth rate on different media and on the modified Melin-Norkrans medium at different pH levels and to analyze ITS rDNA of the Cms01 isolate. Finally, the basidiospore inoculum is used to induce mycorrhizal synthesis. In this study, these sporocarps were collected from a Fagaceae spp., stand located in Jhongpu, Chiayi county. Then, basidiospores were isolated from the sporocarps and examined under light and scanning electron microscopy. Basidiospores were small, globose and possessed secondary spines. Morphological and molecular analyses indicated that isolate Cms01 belongs to Pisolithus orientalis and that it grows optimally on modified Melin-Norkrans medium at a pH of 6.5. The mycorrhizal association experiment showed that roots of colonized seedlings produced the mantle and hartig net characteristic of ectomycorrhizae. These results showed that this strain (Cms01) was a newly recorded species in the fungal flora of Taiwan and it could form a typical ectomycorrhiza with Cyclobalanopsis glauca seedling and it also belonged to an ectomycorrhizal fungus. |