Record ID No. |
6728 |
Author(s) |
Rahman M. H.*, Okubo A., Kawai S. and Sugiyama S. , 2008 |
Affiliation |
*Department of Soil Science, NAtional University, Gazipur, Bangladesh. |
Title |
Assessing Microbial Community in Andisol Differing in Management Practices by Biochemical and Molecular Fingerprinting Methods. |
Source. Vol.(no):Page |
International Journal of Soil Science. 3(1): 1-10. |
Categories |
Mycorrhiza General |
Subjects |
Biochemistry Methodology |
Sub-subjects |
Biodiversity |
Host |
NA |
Organism |
NA |
Country |
Bangladesh |
Abstracts |
Several biochemical and molecular methods are used to investigate the microbial community structure and microbial diversity in soils. Biochemical (Phospholipid fatty acid: PLFA profiles) and molecular (Denaturing gradient gel electrophoresis of polymerase chain reaction amplified DNA: PCR-DGGE) fingerprinting methods were employed to quantify soil microbial community in Andisol differing in management practices. No-tillage (NT) soil had significantly higher microbial biomass carbon and microbial biomass nitrogen than conventional tillage (CT) soil. PLFA for gram-positive bacteria, gram-negative bacteria, aerobes, cyanobacteria and fungi were significantly higher in NT than CT. On the other hand, PLFA for sulfate-reducing bacteria, methane-oxidizing bacteria and mycorrhizae were significantly higher in CT than PD and NT. The total DNA extracted from Andisol with differing management practices ranged from 21.0 to 33.0 μg g-1 soil. Soil DNA yielded from puddling (PD) showed highest amount and NTg showed lowest amount. There were no significant variations in DNA yield obtained from Andisol of CT and NTg. Highest bacterial diversity evaluated by DNA band number in DGGE analysis based on PCR amplification of 16S rDNA fragments was observed in PD and can be arranged as: PD>CT>NT. On the other hand, highest fungal diversity evaluated by DNA band number in DGGE analysis based on PCR amplification of 18S rDNA fragments was observed in NT and can be arranged as: NT>CT>PD. Results indicated that microbial community was responsive to management practices demonstrating their usefulness as indicators of soil quality in temperate Andisol. |