Record ID No. |
6847 |
Author(s) |
Cruz A. F. and Ishii T. , 2011 |
Affiliation |
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Shimogamohangi-cho, Sakyo-ku, Kyoto 606-8522, JAPAN. |
Title |
Arbuscular mycorrhizal fungal spores host bacteria that affect nutrient biodynamics and biocontrol of soilborne plant pathogens. |
Source. Vol.(no):Page |
Biology Open.1: 52-57. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Anatomy,Histology |
Host |
Gigaspora margarita |
Organism |
Bacillus thuringiensis, Bacillus sp., Paenibacillus rhizospherae, Fusarium oxysporum, Rosellinia necatrix, Rhizoctonia solani, Pythium ultimum |
Country |
Japan |
Abstracts |
The aim of this research was to isolate and characterize bacteria from spores of arbuscular mycorrhizal fungi (AMF).
We designated these bacteria ‘probable endobacteria’ (PE). Three bacterial strains were isolated from approximately 500 spores of Gigaspora margarita (Becker and Hall) using a hypodermic needle (diameter, 200 mm). The bacteria were
identified by morphological methods and on the basis of ribosomal gene sequences as Bacillus sp. (KTCIGM01), Bacillus thuringiensis (KTCIGM02), and Paenibacillus rhizospherae (KTCIGM03). We evaluated the effect of these
probable endobacteria on antagonistic activity to the soil-borne plant pathogens (SBPPs) Fusarium oxysporum f. sp. lactucae
MAFF 744088, Rosellinia necatrix, Rhizoctonia solani MAFF 237426, and Pythium ultimum NBRC 100123. We also tested whether these probable endobacteria affected phosphorus solubilization, ethylene production, nitrogenase activity (NA), and stimulation of AMF hyphal growth. In addition, fresh samples of spores and hyphae were photographed using an in situ scanning electron microscope (SEM) (Quanta 250FEG; FEI Co., Japan). Bacterial aggregates (BAs), structures similar to biofilms, could be detected on the surface of hyphae and spores. We demonstrate that using extraction with an ultrathin needle, it is possible to isolate AMF-associated bacterial species
that are likely derived from inside the fungal spores. |