Record ID No. |
76 |
Author(s) |
Troedsson U., Olsson P.A., Jarl-Sunesson C.I. , 2006 |
Affiliation |
Lund University, Department of Cell & Organism Biology, Solvegatan 35B, SE-22362 Lund, Sweden |
Title |
Application of antisense transformation of a barley chitinase in studies of arbuscule formation by a mycorrhizal fungus |
Source. Vol.(no):Page |
Hereditas. 142: 65-72p |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Genetics |
Host |
Barley, Hordeum vulgare |
Organism |
Glomus intraradices |
Country |
Sweden, Europe |
Abstracts |
Barley (Hordeum vulgare L.) plants of two commercial
cultivars were transformed with sense and antisense constructs
of a chitinase class II gene in order to develop a
transformation system for this gene in barley. Transformation of
embryos with the two antisense constructs resulted in ten
regenerated plants, while no plants could be obtained using the
sense construct. The presence of the inserted construct could be
confirmed for six of the plants by PCR analysis. This system was
used to study the role of class II chitinase in the regulation
of mycorrhizal symbiosis. The colonization of two of the
antisense transformants by the arbuscular mycorrhizal fungus
Glomus intraradices was investigated microscopically and by use
of signature fatty acids. The arbuscular incidence increased in
transformed barley, and one transformant supported higher
extraradical mycelium biomass. It is concluded that antisense
transformation of barley could be a useful tool in
investigations on the symbiosis between barley and mycorrhizal
fungi.
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