Record ID No. |
762 |
Author(s) |
Engvild,K.C., Rasmussen,K , 2005 |
Affiliation |
Riso National Laboratory, Plant Research Department, PRD-313, DK-4000, Roskilde, Denmark. |
Title |
Root hair mutants of barley. |
Source. Vol.(no):Page |
Mutation-Breeding-Newsletter-and-Reviews. (1), 12-13 |
Categories |
Mycorrhiza General |
Subjects |
Genetics |
Host |
Barley |
Organism |
n.a. |
Country |
Denmark, Europe |
Abstracts |
Seeds of barley cv. Lux were soaked overnight and thereafter treated with 1.5 mM sodium azide in 0.1 M sodium phosphate buffer, pH 3, for 2.5 h. After rinsing and air-drying, the M2 seeds were sown in the field (Denmark) on the same day. Spikes, 4-6 per M1 plant, were harvested. The mutation frequency was similar to that obtained with other barley cultivars from which low-phytate mutants were isolated. Seeds were germinated for 3-4 days before scoring for root hair mutants. The results showed that root hair mutants can be quite common in plants other than Arabidopsis. The mutants were viable and could be used as genetic markers in investigations of root hair physiology, root architecture, nutrient uptake and the function and importance of mycorrhiza. Some of the mutants showed unstable, variable or poorly visible phenotypes. |