Record ID No. |
827 |
Author(s) |
Serralde O. A. M., Ramirez G. M.M. , 2004 |
Affiliation |
Programa Nacional de Recursos Biofisicos, Corporacion Colombiana de Investigacion Agropecuaria, Centro de Investigacion Tibaitati, Km 14 via Mosquera, Bogota, Colombia. |
Title |
Mycorrhiza populations analysis in corn (Zea mays) cultivated in acid soils under different agronomic treatments |
Source. Vol.(no):Page |
Revista Corpoica. 5(1): 31-40p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Soil plant relations |
Sub-subjects |
Manuring |
Host |
Zea mays |
Organism |
Glomus, Entrophospora and Gigaspora |
Country |
Colombia, South America |
Abstracts |
A study was carried out to evaluate the populations of native arbuscular mycorrhizas (AM) fungi on established crops on acid soils of the Colombian Eastern Plains, for a period of five years (1997-2001). Fungi spores were isolated from the crop-fungi association of two maize cultivars: ICA-Sikuani V-110 and the regional cultivar Clavito. The mycorrhizal behaviour was evaluated under six different organic fertilization treatments, which included green manure (cowpea), chicken manure and a control treatment (no application). From a total of 7924 spores, 24 different types of mycorrhiza were characterized using morphological and molecular analysis. The relation between soil conditions (pH, organic matter contents, P, K, Al+++ and % Al saturation) and AM fungi populations was analysed using a Stepwise Multiple Regression model. According to the regression coefficients obtained (P<0.001 y R2 <=0.83), soil pH and organic matter content were the independent variables that explained most of the variation observed in AM populations. The molecular methodology was based on the Polymerase Chain Reaction (PCR) and the use of specific primers allowed to identify spores from Glomus, Entrophospora and Gigaspora. This methodology also provided the identification of fungi from the Glomus genera in highly colonized maize roots.. |