Record ID No. |
84 |
Author(s) |
Bertini L., Rossi I., Zambonelli A., Amicucci A., Sacchi A., Cecchini M., Gregori G., Stocchi V. , 2006 |
Affiliation |
University of Urbino, Institute of Chemical Biology, Giorgio Fornaini, Via Saffi 2, I-61029 Urbino, PU, Italy |
Title |
Molecular identification of Tuber magnatum ectomycorrhizae in the field |
Source. Vol.(no):Page |
Microbiological Research.161(1): 59-64p. |
Categories |
Ectomycorrhiza |
Subjects |
Genetics |
Host |
EcM |
Organism |
Tuber magnatum, T. maculatum, T. rufum |
Country |
Italy, Europe |
Abstracts |
Tuber ectomycorrhizae in a Tuber magnatum "truffiere", located in Central Italy, were studied using molecular methods. Specifically, RFLP-ITS analyses, ITS sequencing and specific probes hybridization were used to identify 335 Tuber-like ectomycorrhizal morphotypes. Molecular identification was possible even when distinct morphological characteristics were lacking. For the first time, T magnatum ectomycorrhizae and other coexisting Tuber species collected in the field were analysed using molecular tools for unambiguous identification. Although the "truffiere" under investigation yields good harvests of T magnatum fruiting bodies, the percentage of T magnatum ectomycorrhizae found was very low (less than 4.4% of the 335 root tips analysed), whereas the percentages of Tuber maculatum and Tuber rufum were considerably higher (48.9% and 19.0%, respectively). |