Record ID No. |
88 |
Author(s) |
Warren J.A., Covert S.F. , 2004 |
Affiliation |
University of Georgia,Warnell School of Forest Resources,Athens,GA 30602 USA |
Title |
Differential expression of pine and Cronartium quercuum f. sp fusiforme genes in fusiform rust galls |
Source. Vol.(no):Page |
Applied and Environmental Microbiology. 70(1):441-451p. |
Categories |
Mycorrhiza General |
Subjects |
Genetics |
Host |
Pines |
Organism |
n.a. |
Country |
U.S.A., North America |
Abstracts |
Cronartium quercuum f. Sp. Fusiforme is the causative
agent of fusiform rust disease of southern pines in the United
States. This disease is characterized by the formation of woody
branch and stem galls. Differential display was used to identify
pine genes whose expression is altered by C quercuum C sp.
Fusiforme infection and to identify C quercuum f. Sp. Fusiforme
genes that are expressed in fusiform rust galls. Six pine cDNAs
that appeared to be differentially expressed in galled and
healthy stems and 13 C quercuum f. Sp. Fusiforme cDNAs expressed
in galled tissues were identified. A probe that hybridizes
specifically to C quercuum f. Sp. Fusiforme 18S rRNA was used to
estimate that 14% of the total RNA in fusiform rust galls was
from C quercuum f. Sp. Fusiforme. This finding was used to
calibrate gene expression levels in galls when comparing them to
expression levels in uninfected pines or in isolated C quercuum
f. Sp. Fusiforme cultures. According to Northern analysis and
reverse transcriptase PCR analysis, all six of the pine clones
were expressed at lower levels in galls than in healthy tissues. Seven of the nine C. Quercuum f. Sp. Fusiforme clones that were
assayed were expressed at higher levels in galls than in axenic
culture. A number of the cDNAs encode proteins that are similar
to those that play roles in plant development, plant defense, or
fungal stress responses.
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