Record ID No. |
943 |
Author(s) |
Rivera-Becerril,-F., Tuinen, D van., Martin-Laurent,-F., Ashraf-Metwally., Dietz,K.J., Gianinazzi,S., Gianinazzi-Pearson,V , 2005 |
Affiliation |
UMR 1088 INRA/5184 CNRS/U., Bourgogne Plante-Microbe-Environnement, INRA-CMSE, BP 86510, 21065 Dijon Cedex, France. |
Title |
Molecular changes in Pisum sativum L. roots during arbuscular mycorrhiza buffering of cadmium stress |
Source. Vol.(no):Page |
Mycorrhiza. 16(1): 51-60p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Biochemistry |
Host |
Pisum sativum |
Organism |
Glomus intraradices |
Country |
France, Europe |
Abstracts |
Molecular responses to cadmium (Cd) stress were studied in mycorrhizal and non-mycorrhizal Pisum sativum L. cv. Frisson inoculated with Glomus intraradices. Biomass decreases caused by the heavy metal were significantly less in mycorrhizal than in non-mycorrhizal plants. Real-time reverse transcriptase-polymerase chain reaction showed that genes implicated in pathways of Cd detoxification varied in response to mycorrhiza development or Cd application. Expression of a metallothionein-encoding gene increased strongly in roots of Cd-treated non-mycorrhizal plants. Genes encoding gamma -glutamylcysteine synthetase and glutathione (GSH) synthetase, responsible for the synthesis of the phytochelatin (PC) precursor GSH, were activated by Cd in mycorrhizal and non-mycorrhizal plants. Cd stress decreased accumulation of GSH/homoglutathione (hGSH) and increased thiol groups in pea roots, whether mycorrhizal or not, suggesting synthesis of PCs and/or homophytochelatins. An hGSH synthetase gene, involved in hGSH synthesis, did not respond to Cd alone but was activated by mycorrhizal development in the presence of Cd. Transcript levels of a glutathione reductase gene were only increased in non-mycorrhizal roots treated with Cd. Studies of three stress-related genes showed that a heat-shock protein gene was activated in mycorrhizal roots or by Cd and chitinase gene transcripts increased under Cd stress to a greater extent in mycorrhizal roots, whilst a chalcone isomerase gene was only up-regulated by Cd. Results indicate that although heavy metal chelation pathways contribute to Cd stress responses in pea, they may not make a major contribution to Cd tolerance strategies operating in the arbuscular mycorrhizal symbiosis. |