Record ID No. |
975 |
Author(s) |
Fernandez,F., Dell , 2005 |
Affiliation |
Departamento de Biofertilizantes y Nutricion de las Plantas, Instituto Nacional de Ciencias Agricolas, Gaveta Postal 1, San Jose de las Lajas, La Habana, C.P. 32 700, Cuba. |
Title |
Arbuscular mycorrhiza fungal inoculants of Glomus mosseae and Glomus sp.1 in liquid suspension |
Source. Vol.(no):Page |
Cultivos-Tropicales. 26(4): 29-36p. |
Categories |
Arbuscular Mycorrhiza |
Subjects |
Methodology |
Host |
Daucus carota |
Organism |
Glomus mosseae |
Country |
Cuba, N.America |
Abstracts |
Two experiments were conducted to evaluate the fungal viability of Glomus mosseae (INCAM 2) and Glomus sp. (INCAM 4) in liquid suspension, as well their biological characteristics in transformed (Ri TCDNA) carrot rootlets (TCR). Fungal spore viability was determined in liquid suspension and in water, and was evaluated weekly and monthly. The colonization capability of spores stored for 8 months in TCR was determined by measuring spore population, germination average, and colonized root length. Analysis of variance and Turkey test were carried out to compare the media used. Higher spore viability and stability were observed after 8 months, with important spore viability losses in water media. No significant differences were found in the germination ability of the 2 strains up to 30 days of incubation. Higher germination percentages in spores stored in liquid suspension were recorded, which could be due to the strong osmotic stress during the exposure of spore walls in a liquid suspension for 8 months. During the colonization tests, it was found that the spores not only germinated, but a profuse colonization was observed for both strains 14 days later, indicating that liquid suspension was effective in keeping the good functioning levels of the fungi. |