Record ID No. |
99 |
Author(s) |
Guidot A., Debaud J.C., Effosse A., Marmeisse R. , 2004 |
Affiliation |
University Lyon 1, Lab Ecol Microbienne, CNRS, UMR 5557, Batiment A Lwoff,43 Blvd 11 Novembre 1918, F-69622 Villeurbanne, France |
Title |
Below-ground distribution and persistence of an ectomycorrhizal fungus |
Source. Vol.(no):Page |
New Phytologist.161(2):539-547p. |
Categories |
Ectomycorrhiza |
Subjects |
Ecology |
Host |
Pinus pinaster |
Organism |
Hebeloma cylindrosporum |
Country |
France, Europe |
Abstracts |
In most studies, the distribution of the mycelia of
ectomycorrhizal fungi in forest soils has been inferred from
areas occupied by fruit bodies. Here, we investigated the
below-ground spatio-temporal distribution of Hebeloma
cylindrosporum by polymerase chain reaction quantification of a
specific DNA sequence present in DNA extracted from soil.Soil
samples were collected in a Pinus pinaster stand located in a
campsite where fruit bodies of H. cylindrosporum had been mapped
from 1990 to 2000.In samples collected underneath fruit bodies,
DNA of H. cylindrosporum was always detected in large amounts.
However, this DNA was often undetectable in the absence of fruit
body even 1 yr after their disappearance. This result was
supported by the failure to identify mycorrhizas of this species
on seedlings planted in soil samples collected in places where
fruit bodies were present 1 yr before sampling.This pattern
suggests a recolonization of the site each year by H.
cylindrosporum basidiospores in 'receptive' patches of ground
created either by the local elimination of competitors or by
local nutrient enrichments, which could frequently occur in a
campsite. Our results demonstrate that an ectomycorrhizal
species can be completely eliminated from the roots within 1 yr
and does not necessarily contribute to the next generation of
mycorrhizas
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